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Your Position: Home > Protein > Claudin-6 > CL6-H52M3

Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag (Nanodisc-pro)

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  • Synonym
    Claudin-6,CLDN6
  • Source
    Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag(CL6-H52M3) is expressed from human 293 cells (HEK293). It contains AA Ala 2 - Val 220 (Accession # P56747).
    Predicted N-terminus: Met
  • Molecular Characterization
    Claudin-6 Structure

    This protein carries a flag tag at the N-terminus, followed by a polyhistidine tag, and a GFP tag at the C-terminus.

    The protein has a calculated MW of 54.0 kDa. The protein migrates as 50-53 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

    Nanodiscs are a new class of model membranes that are being used to solubilize and study a range of integral membrane proteins and membrane-associated proteins. The Nanodisc bilayer is bounded by an amphipathic scaffold coat that confers enhanced stability and a narrow particle size distribution.

    The nanodisc-pro is directly extracted from cell membrane in a water-soluble form using an amphipathic scaffold followed by affinity chromatography, and thus, it conserves a patch of native membrane around them.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >85% as determined by SDS-PAGE.

  • Formulation

    Supplied as 0.2 μm filtered solution in 20 mM HEPES, 150 mM NaCl, pH7.5 with glycerol as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. The product MUST be stored at -70°C or lower upon receipt;
    2. -70°C for 3 months under sterile conditions.
SDS-PAGE
Claudin-6 SDS-PAGE

Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 85% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 Claudin-6 ELISA

Immobilized Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag (Cat. No. CL6-H52M3) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-Human Claudin 6 antibody, Human IgG1 with a linear range of 0.05-2 ng/mL (QC tested).

Bioactivity-SPR
 Claudin-6 SPR

Anti-Claudin-6 Antibody, Human IgG1 captured on Protein A Chip can bind Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag (Cat. No. CL6-H52M3) with an affinity constant of 1.52 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

Bioactivity-FACS
 Claudin-6 FACS

Flow cytometric analysis of overexpressing Anti-Claudin-6(BBz) CAR-293 cells staining with Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag (Cat. No. CL6-H52M3) (2e5 of Anti-Claudin-6(BBz) CAR-293 cells were stained with 100 μL of 5μg/mL of Fluorescent Human Claudin-6 Full Length Protein, Flag,His Tag,GFP Tag), compared with negative control protein analyzed with FACS (QC tested). ​

  • Background
    Tight junctions represent one mode of cell-to-cell adhesion in epithelial or endothelial cell sheets, forming continuous seals around cells and serving as a physical barrier to prevent solutes and water from passing freely through the paracellular space. These junctions are comprised of sets of continuous networking strands in the outwardly facing cytoplasmic leaflet, with complementary grooves in the inwardly facing extracytoplasmic leaflet. Claudin-6, also knows as CLDN6, is a multipass transmembrane protein in the Claudin family. Claudin-6 is expressed by epithelial cells where it participates in tissue development and the maintenance of tight junction integrity. And it is one of the entry cofactors for hepatitis C virus. The methylation of CLDN6 may be involved in esophageal tumorigenesis. The gene of CLDN6 is adjacent to another family member CLDN9 on chromosome 16.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:8 Details
  • Latest Research Phase:Phase 2 Clinical

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