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The Monoclonal Anti-Mouse IgG Antibody FA-Labeled is a universal tool for TR-FRET assays that can bind mouse IgG or mFc-tagged proteins. This product uses specific anti-mouse IgG antibody labeled with fluorescent dyes and it can be used in combination with other Donors covalently conjugated with Eu³⁺ chelate. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.
| Source | This Monoclonal Anti-Mouse IgG Antibody is a rabbit monoclonal antibody which recognizes mouse immunoglobulins with high affinity. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Physical Appearance | Powder |
| Storage (unopened) | -20°C to -70°C |
| Storage (opened) | -70°C |
| Unit Size | 1000 tests & 5000 tests & 20000 tests |
| Technology | TR-FRET |
| Intended Use | For binding the mouse IgG antibody or mouse Fc tagged protein as acceptor in TR-FRET Assay |
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
In the TR-FRET assay, Streptavidin Protein Europium chelate is used as the donor and Monoclonal Anti-Mouse IgG Antibody FA-Labeled (Cat. No. FRT-TA03) as the acceptor. Mix Human CD3E&CD3D Heterodimer Protein, His,Avitag&Tag Free (Cat. No. CDD-H82W6) at 20 ng/well can bind Monoclonal Anti-Human CD3 Antibody, Mouse IgG2a (Clone: OKT3), premium grade (Cat. No. CDE-M120a) with a linear range of 8-0.125 μg/mL (QC tested).
The TR-FRET assay shows that Monoclonal Anti-Mouse IgG Antibody FA-Labeled (Cat. No. FRT-TA03) is stable at 37°C for 50 hours.
The TR-FRET assay shows that Monoclonal Anti- Mouse IgG Antibody FA-Labeled (Cat. No. FRT-TA03) is stable after freezing and thawing 3 times.
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