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The Monoclonal Anti-His Tag Antibody, Human IgG1 FA-Labeled is a universal tool for TR-FRET assays that can bind His-tagged proteins. This product uses specific anti-His antibody labeled with fluorescent dyes and it can be used in combination with other Donors covalently conjugated with Eu³⁺ chelate. When the Donor and Acceptor come into close proximity (within a distance of less than 10 nm), a FRET reaction occurs: the 620 nm signal emitted by the Donor upon excitation by a specific light source is received by the Acceptor, which then emits a 665 nm signal.
| Source | This Monoclonal Anti-His Tag Antibody, Human IgG1 is a chimeric monoclonal antibody recombinantly expressed from HEK293, which combines the variable region of a mouse monoclonal antibody with Human constant domain. |
| Formulation | Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant. |
| Physical Appearance | Powder |
| Storage (unopened) | -20°C to -70°C |
| Storage (opened) | -70°C |
| Unit Size | 1000 tests & 5000 tests & 20000 tests |
| Technology | TR-FRET |
| Intended Use | For binding the his-tagged protein as acceptor in TR-FRET Assay |
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
In the TR-FRET assay, Streptavidin Protein Europium chelate is used as the donor and Monoclonal Anti-His Tag Antibody, Human IgG1 FA-Labeled (Cat. No. FRT-TA04) as the acceptor. Mix Biotinylated Human PD-1, Fc, Avi tag (Cat. No. PD1-H82F1) at 25 ng/well can bind Human PD-L1, His Tag (Cat. No. PD1-H5229) with a linear range of 20-0.3125 μg/mL (QC tested).
In the TR-FRET assay, Streptavidin Protein Europium chelate is used as the donor and Monoclonal Anti-His Tag Antibody, Human IgG1 FA-Labeled (Cat. No. FRT-TA04) as the acceptor. Inhibition Assay of interaction of Biotinylated Human PD-1, Fc, Avi tag (Cat. No. PD1-H82F1) and Human PD-L1, His Tag (Cat. No. PD1-H5229) by Monoclonal Anti-Human PD-1 Antibody, Human IgG4 with a typical IC50 of 3.358 μg/mL.
The TR-FRET assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1 FA-Labeled (Cat. No. FRT-TA04) is stable at 37°C for 50 hours.
The TR-FRET assay shows that Monoclonal Anti-His Tag Antibody, Human IgG1 FA-Labeled (Cat. No. FRT-TA04) is stable after freezing and thawing 5 times.
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