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The kit is developed for quantitative detection of natural and recombinant mouse IL-6 in serum, plasma and cell culture supernatants.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | Mouse IL-6 |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Mouse |
| Sensitivity | 10 pg/mL |
| Assay Time | 2.75 hr |
| Sample volume | 50 μL |
| Range | 18.75 pg/mL-600 pg/mL |
| Sample Type | For the quantitative determination of mouse IL-6 in serum, plasma and cell supernatant. |
| NIBSC Code | 93/730, NIBSC/WHO (93/730) approximate value (U/mL) = 0.25 x ACRO Mouse IL-6 (pg/mL) |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of mouse IL-6 is less than 10 pg/mL.
Serum Sample: The mice in the experimental group were injected with 15 μg or 30 μg of LPS and bled 4 hours after injection. In contrast, those in the control group received an equivalent volume of normal saline (NS). Serum samples were collected from all mice, and IL-6 concentrations were quantitatively measured using standardized immunoassays.
Ten replicates of each of three samples containing different Mouse IL-6 concen-trations were tested in one assay. Acceptable criteria: CV＜10%.
Three samples containing different concentrations of Mouse IL-6 were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant IL-6 was spiked into three mouse serum samples, and then analyzed. The average recovery of IL-6 for serum samples is 88.7%.
| ID | Components | Size |
| CEA254-C01 | Pre-coated Anti-mouse-IL-6 Antibody Microplate | 1 plate |
| CEA254-C02 | Mouse IL-6 Standard | 6 ng×2 |
| CEA254-C03 | Biotin-Anti-mouse-IL-6 Antibody Con. Solution | 100 μL |
| CEA254-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA254-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA254-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA254-C07 | 20×Washing Buffer | 50 mL |
| CEA254-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA254-C09 | Substrate Solution | 12 mL |
| CEA254-C10 | Stop Solution | 6 mL |
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