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Your Position: Home > Kits > IL-5 > FCSh-014

Human IL-5 Detection Kit (Flow Cytometry Bead Assay)

For research use only.

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    Product Specifications
    Assay TypeFACS
    AnalyteIL-5
    Format50 tests×2
    ReactivityHuman
    Regulatory StatusRUO
    Sensitivity1.15 pg/mL
    Standard Curve Range9.8~5000 pg/mL
    Assay Time3 hr
    Suitable Sample TypeHuman serum, Human plasma, Cell culture supernatants
    Sample volume15 μL
  • Product Overview
    Multiplex Bead Assay platform allows flow cytometry based on beads with different size and color to quantity multiple soluble cell release factor from a single sample. The performance of this platform has been optimized for specific analysis in cell culture supernatants, plasma and serum samples. Compared to traditional single-analyte methods, it offers significant advantages, including lower sample volume requirements, simpler operation, more broad dynamic range and so on.

    We offer a range of individual reagents for each factor, giving you the flexibility to select and combine them according to your specific research requirements.

  • Application

    This Panel (Flow Cytometry Bead Assay) gives quantitative results of Interleukin-5 (IL-5) with a sample testing. The performance of this panel has been optimized for specific analysis of cytokines.

    It is for research use only.

  • Storage
    1. Unopened buffer should be stored at 2℃-8℃ upon receiving.

    2. Find the expiration date on the outside packaging and do not use reagents past their expiration date.

    3. The shelf life is 30 days from the date of opening.

    Materials Provided
    IDComponentsSize
    FMBc014-02Anti-human IL-5 Antibody-coupled APC-beads-C0350 tests
    FABm030-01PE labeled Monoclonal Anti-Human IL-5 Antibody, Rat IgG1 0.25 mL
Typical Data Please refer to DS document for the assay protocol.
 IL-5 TYPICAL DATA

A double logarithmic fitting curve was plotted for IL-10. The present curve of each cytokines was ranging from 9.8 pg/ml to 5000 pg/mL.

Bioactivity-FACS Please refer to DS document for the assay protocol.
 IL-5 FACS

A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC fluorescence intensity (middle), and fluorescence signal in propotion to concentration of the analyte was measured for each cytokine in PE channel (right). Data was generated on Beckman Cytoflex S Flow Cytometer, equipped with blue laser (488 nm) and red laser (640 nm).

  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:2 Details
  • Number of Drugs in Clinical Trials:8 Details
  • Latest Research Phase:Approved

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