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Your Position: Home > > MMAE > APA-01

Anti-MMAE Antibody Screening Panel

Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells 

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    Components
    Cat.No.Clone
    MME-MY2209M1H09
    MME-MY2210M1G04
    MME-MY2211M1D12
    MME-MY2198aM1H05
  • Specificity

    Specifically recognizes MMAE and does not recognize MMAF.

  • Source
    This panel contains four anti-MMAE monoclonal antibodies, suitable for pharmacokinetic (PK) detection of ADC with MMAE as the payload. All the anti-MMAE monoclonal antibodies are recombinantly expressed from HEK293, can be used as a capture antibody for detecting conjugated antibodies in ELISA assay.
  • Isotype
    Rabbit IgG | Rabbit Kappa
  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-ELISA
 MMAE ELISA

Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H09) (Cat. No. MME-MY2209) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum with a linear range of 0.016-0.98 μg/mL, and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).

 MMAE ELISA

Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1G04) (Cat. No. MME-MY2210) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum with a linear range of 0.016-0.98 μg/mL, and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).

 MMAE ELISA

Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1D12) (Cat. No. MME-MY2211) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum with a linear range of 0.016-0.98 μg/mL, and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).

 MMAE ELISA

Immobilized Monoclonal Anti-MMAE specific Antibody, Rabbit IgG (M1H05) (Cat. No. MME-MY2198A) at 5 μg/mL, add Disitamab Vedotin (RC48) in the 100% Human Serum with a linear range of 0.016-0.98 μg/mL, and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).

  • Background
    Monomethyl auristatin E (MMAE) is a synthetic derivative of dolastatin 10 and functions as a potent mitotic inhibitor by inhibiting tubulin polymerization. MMAE is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several cancer types. Anti-MMAE antibody is a rabbit monoclonal antibody. The anti-MMAE antibody is a useful reagent in PK assay to determine conjugated antibodies.
  • Clinical and Translational Updates

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