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Your Position: Home > > MMAF > APA-02

Anti-MMAF Antibody Screening Panel

Antibody Internalization Detection Reagent (IGG-PZF2001) monitors endocytosis in live cells 

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    Components
    Cat.No.Clone
    MMF-MY22131M1G10
    MMF-MY2220M1B12
    MMF-MY22141M1E12
    MMF-MY2219M1E04
  • Specificity

    Specifically recognizes MMAF and does not recognize MMAE.

  • Source
    This panel contains four anti-MMAF monoclonal antibodies, suitable for pharmacokinetic (PK) detection of ADC with MMAF as the payload. All the anti-MMAF monoclonal antibodies are recombinantly expressed from HEK293, can be used as a capture antibody for detecting conjugated antibodies in ELISA assay.
  • Isotype
    Rabbit IgG | Rabbit Kappa
  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-ELISA
 MMAF ELISA

Immobilized IgG1-MMAF at 0.1 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (M1E04) (Cat. No. MMF-MY2219) with a linear range of 0.06-0.97 ng/mL (QC tested).

 MMAF ELISA

Immobilized IgG1-MMAF at 0.2 μg/mL (100 μL/well) can bind Monoclonal Anti-MMAF specific Antibody, Rabbit IgG (1M1E12) (Cat. No. MMF-MY2214) with a linear range of 0.06-0.97 ng/mL (QC tested).

  • Background
    Monomethyl auristatin F (MMAF) is a potent mitotic inhibitor that inhibits tubulin polymerization. MMAF is widely used as a cytotoxic component of antibody-drug conjugates (ADCs) to treat several cancer types. Anti-MMAF antibody is a rabbit monoclonal antibody specifically reacts with MMAF without MMAE, which is more sensitive than mouse antibody. The anti-MMAF antibody is a useful reagent in PK assay to determine conjugated antibodies.
  • Clinical and Translational Updates

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