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Your Position: Home > Protein > HGF R > MET-H82E4

Biotinylated Human HGF R / c-MET Protein, His,Avitag™

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  • Synonym
    MET, AUTS9, HGFR, RCCP2, c-Met
  • Source
    Biotinylated Human HGF R Protein, His,Avitag(MET-H82E4) is expressed from human 293 cells (HEK293). It contains AA Glu 25 - Lys 567 (Accession # P08581-1).
    Predicted N-terminus: Glu 25
  • Molecular Characterization
    HGF R Structure

    The protein has a calculated MW of 65.2 kDa. The protein migrates as 38-48 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Labeling
    Biotinylation of this product is performed using Avitag™ technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
  • Protein Ratio
    Passed as determined by the HABA assay / binding ELISA.
  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >90% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
HGF R SDS-PAGE

Biotinylated Human HGF R Protein, His,Avitag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 HGF R ELISA

Immobilized Biotinylated Human HGF R Protein, His,Avitag (Cat. No. MET-H82E4) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human HGF, Fc Tag (Cat. No. HGF-H5253) with a linear range of 0.08-2 ng/mL (QC tested).

  • Background
    This gene encodes a member of the receptor tyrosine kinase family of proteins and the product of the proto-oncogene MET. The encoded preproprotein is proteolytically processed to generate alpha and beta subunits that are linked via disulfide bonds to form the mature receptor. Further processing of the beta subunit results in the formation of the M10 peptide, which has been shown to reduce lung fibrosis. Binding of its ligand, hepatocyte growth factor, induces dimerization and activation of the receptor, which plays a role in cellular survival, embryogenesis, and cellular migration and invasion. Mutations in this gene are associated with papillary renal cell carcinoma, hepatocellular carcinoma, and various head and neck cancers. Amplification and overexpression of this gene are also associated with multiple human cancers
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:9 Details
  • Number of Drugs in Clinical Trials:59 Details
  • Latest Research Phase:Approved

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