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Your Position: Home > Protein > IL-1 alpha > ILA-H5116

Human IL-1 alpha / IL-1F1 Protein, premium grade

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  • Synonym
    IL1A,IL-1 ALPHA,IL1F1,IL1α
  • Source
    Human IL-1 alpha Protein, premium grade(ILA-H5116) is expressed from E. coli cells. It contains AA Ser 113 - Ala 271 (Accession # P01583-1).
    Predicted N-terminus: Ser 113
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization
    IL-1 alpha Structure

    This protein carries no "tag".

    The protein has a calculated MW of 10.0 kDa. The protein migrates as 18 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method / rFC method.
  • Host Cell Protein
    <0.5 ng/µg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Sterility
    Negative
  • Mycoplasma
    Negative
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 24 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-1 alpha SDS-PAGE

Human IL-1 alpha Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
IL-1 alpha MALS images

The purity of Human IL-1 alpha Protein, premium grade (Cat. No. ILA-H5116) is more than 95% and the molecular weight of this protein is around 15-22 kDa verified by SEC-MALS.

Bioactivity-CELL BASE
 IL-1 alpha CELL

Human IL-1 alpha Protein, premium grade (Cat. No. ILA-H5116) induced NF-κB Reporter activity in HEK293 cells. The EC50 of the effect is 0.838 ng/mL (Routinely tested).

Bioactivity-ELISA
 IL-1 alpha ELISA

Immobilized Human IL-1 alpha Protein, premium grade (Cat. No. ILA-H5116) at 1 μg/mL (100 μL/well) can bind Human IL-1 RII, Fc Tag (Cat. No. IL2-H4256) with a linear range of 0.2-13 ng/mL (QC tested).

  • Background
    Interleukin-1 alpha (IL1a) is also known as IL-1A, IL1, IL1-ALPHA,IL1F1,and is a cytokine of the interleukin-1 family. IL1a possesses a wide spectrum of metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. IL-1α is constitutively produced by epithelial cells. It is found in substantial amounts in normal human epidermis and is distributed in a 1:1 ratio between living epidermal cells and stratum corneum.The constitutive production of large amounts of IL-1α precursor by healthy epidermal keratinocytes interfere with the important role of IL-1α in immune responses, assuming skin as a barrier, which prevents the entry of pathogenic microorganisms into the body. In vitro, IL-1α possesses biological effect on cells in the picomolar to femtomolar range. In vivo, Shortly after an onset of an infection into organism, IL-1α activates a set of immune system response processes.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:7 Details
  • Latest Research Phase:Approved

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