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Your Position: Home > Protein > IL-21 > IL1-H5113

Human IL-21 Superior Stable Mutant Protein, premium grade

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  • Synonym
    IL21,Za11,Interleukin-21
  • Product Details
    Our Recombinant Human IL-21 Superior Stable Mutant Protein, developed via an AI-modified and experimentally validated closed-loop efficient protein design platform, is the optimal IL-21 mutant with superior thermal stability and activity compared to wild-type IL-21, making it more suitable for various immune cell culture processes. This reduces medium replacement frequency, lowers contamination risks from frequent operations, and saves costs/time for immune cell culture by minimizing reagent consumption and labor, especially in large-scale applications.
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization

    This protein carries no "tag".

    The protein has a calculated MW of 15.6 kDa. The protein migrates as 16 kDa±3 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method / rFC method.
  • Host Cell Protein
    <0.5 ng/µg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Sterility
    Negative
  • Mycoplasma
    Negative
  • Purity

    >95% as determined by SDS-PAGE.

    >95% as determined by SEC-HPLC.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
IL-21 SDS-PAGE

Human IL-21 Superior Stable Mutant Protein, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-HPLC
IL-21 SEC-HPLC

The purity of Human IL-21 Superior Stable Mutant Protein, premium grade (Cat. No. IL1-H5113) was greater than 95% as determined by SEC-HPLC.

Heat Stability
 IL-21 HEAT STABILITY

Recombinant Human IL-21 stability studies in the T culture medium. Human IL-21 Superior Stable Mutant Protein, premium grade (Cat. No. IL1-H5113) or wild-type (WT) Human IL-21 Protein, premium grade (Cat. No. IL1-H5114), or Company M Human IL-21 Protein were added in the T cell medium at an initial concentration of 22 ng/mL, respectively. IL-21 concentration tested with resDetect™ Human Interleukin-21 (IL-21) ELISA Kit (Cat. No. CRS-A010). Following 3 days of treatment at 37°C, Recombinant Human IL-21 Superior Stable Mutant Protein retained the concentration 70%, demonstrating its enhanced thermal stability. In contrast, the WT Recombinant Human IL-21 Protein exhibited significant concentration loss, indicating inferior thermal stability.

Bioactivity-CELL BASE
 IL-21 CELL

Human IL-21 Superior Stable Mutant Protein, premium grade (Cat. No. IL1-H5113) stimulates secretion of IFN-γ by NK-92 human natural killer lymphoma cells stimulated with Human IL-15, premium grade (Cat. No. IL5-H4117), The specific activity of Human IL-21 Superior Stable Mutant Protein, premium grade is > 4.00 x 10^5 U/mg (QC tested).

Bioactivity-ELISA
 IL-21 ELISA

Immobilized Human IL-21 Superior Stable Mutant Protein, premium grade (Cat. No. IL1-H5113) at 5 μg/mL (100 μL/well) can bind Human IL-21 R, Fc Tag with a linear range of 0.5-8 ng/mL (QC tested).

  • Background
    Interleukin-21 (IL-21) is a secreted protein which belongs to the IL-15 / IL-21 family. Interleukin-21 / IL-21 belongs to a family of cytokines that bind to a composite receptor consisting of a private receptor (IL21R) and the common cytokine receptor gamma chain (gamma(C)). Interleukin-21 / IL-21 impacts a number of cell types, including CD8+ memory T cells, NK cells and subsets of CD4 memory T cells. The IL-21R is widely distributed on lympho-haematopoietic cells. IL-21 is a pleiotropic cytokine produced by CD4+ T cells in response to antigenic stimulation. Its action generally enhances antigen-specific responses of immune cells. IL-21 promotes the anti-tumor activity of CD8+ T-cells and NK cells. IL-21 exerts its effect through binding to a specific type I cytokine receptor, IL-21R, which also contains the γ chain (γc) found in other cytokine receptors including IL-2, IL-4, IL-7, IL-9 and IL-15. The IL-21/IL-21R interaction triggers a cascade of events which includes activation of the tyrosine kinases JAK1 and JAK3, followed by activation of the transcription factors STAT1 and STAT3.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:0 Details
  • Number of Drugs in Clinical Trials:4 Details
  • Latest Research Phase:Phase 2 Clinical

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