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Your Position: Home > Kits > TNF-alpha > CEA-M255

Mouse TNF-alpha ELISA Kit

For research use only.
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  • Application

    The kit is developed for quantitative detection of natural and recombinant mouseTNF-alpha in serum, plasma and cell culture supernatants.

    It is for research use only.

  • Workflow
    TNF-alpha Workflow
  • Product Specifications
    Assay TypeSandwich-ELISA
    AnalyteMouse TNF-alpha
    Format96-wells plate breakable into 12 x 8 wells strips
    ReactivityMouse
    Sensitivity4 pg/mL
    Assay Time2.75 hr
    Sample volume50 μL
    Range7.81 pg/mL-500 pg/mL
    Sample TypeFor the quantitative determination of mouse TNF-alpha in serum, plasma and cell supernatant.
    NIBSC Code88/532, NIBSC/WHO (88/532) approximate value (U/mL) = 0.2 x ACRO Mouse TNF-α (pg/mL)

    Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.

  • Storage
    Keep the unopened kit stored at 2-8 °C. Avoid using the kit beyond its expiration date. The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
Typical Data Please refer to DS document for the assay protocol.
TNF-alpha TYPICAL DATA

For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of mouse TNF-alpha is less than 4 pg/mL.

Validation
Intra-Assay Statistics

Ten replicates of each of three samples containing different TNF-α concentrations were tested in one assay. Acceptable criteria: CV＜10%.

TNF-alpha INTRA-ASSAY STATISTICS
Inter-Assay Statistics

Three samples containing different concentrations of TNF-α were tested in in-dependent assays. Acceptable criteria: CV<15%.

TNF-alpha INTER-ASSAY STATISTICS
Recovery

Recombinant TNF-alpha was spiked into three mouse serum samples, and then analyzed. The average recovery of TNF-alpha for serum samples is 90.1.%.

TNF-alpha RECOVERY
Materials Provided
IDComponentsSize
CEA255-C01Pre-coated Anti-mouse TNF-alpha Antibody Microplate1 plate
CEA255-C02Mouse TNF-alpha Standard5 ng×2
CEA255-C03Biotin-Anti-mouse TNF-alpha Antibody Con. Solution100 μL
CEA255-C04Biotin-Antibody Dilution Buffer8 mL
CEA255-C05Streptavidin-HRP Con. Solution500 μL
CEA255-C06Streptavidin-HRP Dilution Buffer15 mL
CEA255-C0720×Washing Buffer50 mL
CEA255-C08Sample Dilution Buffer15 mL×2
CEA255-C09Substrate Solution12 mL
CEA255-C10Stop Solution6 mL
  • Clinical and Translational Updates

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