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Your Position: Home > Antibody > Nucleoprotein > NUN-MY2301

Monoclonal Anti-Rabies virus (strain CVS-11) Nucleoprotein Antibody, Mouse IgG1 (7B7) (MALS verified)

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  • Source

    Monoclonal Anti-Rabies virus (strain CVS-11) Nucleoprotein Antibody, Mouse IgG1 (7B7) is a Mouse monoclonal antibody recombinantly expressed from HEK293 cells.

  • Clone

    7B7

  • Species

    Mouse

  • Isotype

    Mouse IgG1 | Mouse Kappa

  • Conjugate

    Unconjugated

  • Antibody Type

    Recombinant Monoclonal

  • Reactivity

    Virus

  • Immunogen

    Recombinant Rabies virus (strain CVS-11) Nucleoprotein is expressed from Baculovirus-Insect cells

  • Specificity

    Specifically recognizes Rabies virus (strain CVS-11) Nucleoprotein.

  • Application
    ApplicationRecommended Usage
    ELISA0.06-8 ng/mL
  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Purification

    Protein A purified / Protein G purified

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Nucleoprotein SDS-PAGE

Monoclonal Anti-Rabies virus (strain CVS-11) Nucleoprotein Antibody, Mouse IgG1 (7B7) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Nucleoprotein MALS images

The purity of Monoclonal Anti-Rabies virus (strain CVS-11) Nucleoprotein Antibody, Mouse IgG1 (7B7) (Cat. No. NUN-MY2301) is more than 90% and the molecular weight of this protein is around 130-160 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Nucleoprotein ELISA

Immobilized Rabies virus (strain CVS-11) Nucleoprotein, His Tag (Cat. No. NUN-R55H4) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Rabies virus (strain CVS-11) Nucleoprotein Antibody, Mouse IgG1 (7B7) (Cat. No. NUN-MY2301) with a linear range of 0.06-1 ng/mL (QC tested).

 Nucleoprotein ELISA

Immobilized Rabies virus (strain ERA) Nucleoprotein, His Tag (Cat. No. NUN-R55H3) at 1 μg/mL (100 μL/well) can bind Monoclonal Anti-Rabies virus (strain CVS-11) Nucleoprotein Antibody, Mouse IgG1 (7B7) (Cat. No. NUN-MY2301) with a linear range of 0.008-0.5 μg/mL (Routinely tested).

  • Background
    Rabies virus (RABV), scientific name Rabies lyssavirus, is a deadly neurotropic virus that causes rabies in humans and animals. Rabies virus has an extremely wide host range and its transmission most often occur through the saliva of animals. Without intervention prior to disease progression, rabies has the highest case fatality of any infectious disease. RABV contains a single-stranded negative-sense RNA genome that encodes five structural proteins: nucleoprotein (N), phosphoprotein (P), matrix protein (M), glycoprotein (G), and RNA-dependent RNA polymerase (L). RABV N protein is 450 amino acids long and serves the critical function of tightly packaging the RNA genome into an RNase-resistant core. Encapsidation of the genomic RNA by newly synthesized N is believed to switch viral RNA from transcription to replication. Furthermore, N is a major antigen for RABV to stimulate Th cells and antibody production.
  • Clinical and Translational Updates

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