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Your Position: Home > Protein > ROBO4 > RO4-M52H3

Mouse ROBO4 Protein, His Tag (MALS verified)

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  • Synonym
    ROBO4,ECSM4,MRB
  • Source
    Mouse ROBO4 Protein, His Tag(RO4-M52H3) is expressed from human 293 cells (HEK293). It contains AA Leu 28 - Trp 480 (Accession # Q8C310-1).
    Predicted N-terminus: Leu 28
  • Molecular Characterization
    ROBO4 Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 51.1 kDa. The protein migrates as 65-95 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Endotoxin
    Less than 1.0 EU per μg by the LAL method / rFC method.
  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
ROBO4 SDS-PAGE

Mouse ROBO4 Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
ROBO4 MALS images

The purity of Mouse ROBO4 Protein, His Tag (Cat. No. RO4-M52H3) is more than 90% and the molecular weight of this protein is around 50-75 kDa verified by SEC-MALS.

  • Background
    Predicted to enable cell-cell adhesion mediator activity. Involved in establishment of endothelial barrier. Located in extracellular exosome. Implicated in aortic valve disease 3. Biomarker of pre-eclampsia and thyroid gland carcinoma.
  • Clinical and Translational Updates

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