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This protein carries an Avi tag (Avitag™) and a Flag tag at the N-terminus followed by a polyhistidine tag at the C-terminus. The protein has a calculated MW of 30.7 kDa. The protein migrates as 33-35 kDa and 37 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
>90% as determined by SEC-MALS.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Human IGFBP-4 Protein, Avitag,Flag&His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
The purity of Biotinylated Human IGFBP-4 Protein, Avitag,Flag&His Tag (Cat. No. IG4-H82D3) is more than 90% and the molecular weight of this protein is around 27-42 kDa verified by SEC-MALS.
Immobilized Human IGF-II, Fc Tag (Cat. No. IG2-H4260) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGFBP-4 Protein, Avitag,Flag&His Tag (Cat. No. IG4-H82D3) with a linear range of 0.1-8 ng/mL (QC tested).
Immobilized Human IGF-I, Fc Tag (Cat. No. IG1-H4269) at 5 μg/mL (100 μL/well) can bind Biotinylated Human IGFBP-4 Protein, Avitag,Flag&His Tag (Cat. No. IG4-H82D3) with a linear range of 0.06-4 ng/mL (Routinely tested).
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