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Your Position: Home > Protein > Spike protein > SPN-S52Hx

SARS coronavirus SZ1 Spike Trimer Protein (HT74-75R, R667A, KV968-969PP, E1163K, L1179F), His Tag (MALS verified)

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  • Synonym
    Spike,S protein,Spike glycoprotein,S glycoprotein
  • Source
    SARS coronavirus SZ1 Spike Trimer Protein, His Tag(SPN-S52Hx) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAS00003.1 (HT74-75R, R667A, KV968-969PP, E1163K, L1179F)).
    Predicted N-terminus: Ser 14
  • Molecular Characterization

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 136.4 kDa. The protein migrates as 140-180 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.1 M Sodium citrate, pH5.5 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Spike protein SDS-PAGE

SARS coronavirus SZ1 Spike Trimer Protein, His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Spike protein MALS images

The purity of SARS coronavirus SZ1 Spike Trimer Protein, His Tag (Cat. No. SPN-S52Hx) is more than 90% and the molecular weight of this protein is around 510-560 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Spike protein ELISA

Immobilized Human ACE2, Fc Tag (Cat. No. AC2-H5257) at 5 μg/mL (100 μL/well) can bind SARS coronavirus SZ1 Spike Trimer Protein, His Tag (Cat. No. SPN-S52Hx) with a linear range of 0.004-0.125 μg/mL (QC tested).

  • Background
    The spike protein is a large type I transmembrane protein containing two subunits, S1 and S2. S1 mainly contains a receptor binding domain (RBD), which is responsible for recognizing the cell surface receptor. S2 contains basic elements needed for the membrane fusion.The S protein plays key parts in the induction of neutralizing-antibody and T-cell responses, as well as protective immunity.
  • Clinical and Translational Updates

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Drug Development Status

  • Number of Launched Drugs:1 Details
  • Number of Drugs in Clinical Trials:1 Details
  • Latest Research Phase:Approved

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