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Your Position: Home > Antibody > G4S linker > G4S-CY25P1

Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), CF (carrier free), premium grade

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  • Source
    Monoclonal Anti-G4S linker Antibody, Rabbit IgG (016), premium grade is a rabbit monoclonal antibody recombinantly expressed from human 293 cells (HEK293), it shows superior performance in flow cytometry assays with higher affinity and better specificity.
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Isotype
    Rabbit IgG | Rabbit Kappa
  • Conjugate

    Unconjugated

  • Specificity
    It can specifically recognize proteins or ScFv containing (G3S)n or (G4S)n linkers, where n = 2, 3, 4... That is, it can recognize linkers with a minimum length of 2 repeats for both (G3S)n and (G4S)n.
  • Endotoxin
    Less than 0.01 EU per μg by the LAL method / rFC method.
  • Protein A
    <5 ppm of protein tested by ELISA.
  • Host Cell Protein
    <0.5 ng/μg of protein tested by ELISA.
  • Host Cell DNA
    <0.02 ng/μg of protein tested by qPCR.
  • Sterility
    Negative
  • Mycoplasma
    Negative
  • Formulation

    Supplied as 0.2 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Shipping

    This product is supplied and shipped with dry ice, please inquire the shipping cost.

  • Storage

    For long term storage, the product should be stored at liquid state at -20℃ or below.

    This product is stable after storage at:

    1. -20℃ or below for 24 months under sterile conditions;
    2. 2-8℃ for 12 months under sterile conditions.
Bioactivity-FACS
 G4S linker FACS

Flow cytometric analysis of Anti-MSLN CAR-293 cells staining with Monoclonal Anti-G4S Linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-CY25P1) at 3 µg/mL (0.3 µg corresponds to labeling of 1e6 cells in a final volume of 100 µL) and secondary antibody. PE signal was used to evaluate the binding activity (QC tested).

 G4S linker FACS

Non-specificity of Monoclonal Anti-G4S Linker Antibody, Rabbit IgG (016), premium grade (Cat. No. G4S-CY25P1) binding to CD3+ cells present in human PBMC. 5e5 of human PBMCs were simultaneously stained with FITC-labeled anti-CD3 antibody and Monoclonal Anti-G4S Linker Antibody (016) (0.3 µg corresponds to labeling of 5e5 cells in a final volume of 100 µL) and washed and then analyzed with FACS. Both FITC and PE positive signals was used to evaluate the non-specific binding activity to human CD3+ cells (QC tested).

  • Background
    The poly-Glycine-Serine (G4S) linker is a type of flexible, unstructured synthetic peptide linker sequence often leveraged to connect the variable heavy (VH) domain and variable light (VL) domain of single-chain variable fragments (scFvs) and chimeric antigen receptors (CARs) that utilize an extracellular domain scFv for target antigen recognition. The linker itself consists of a core pentapeptide sequence, Gly-Gly-Gly-Gly-Ser, that is repeated and commonly found as either a 15-mer (G4S)3 or20-mer(G4S) 4 within scFv-based CARs and scFv fragments. The linker sequence length plays a role in controlling scFv stability and the noncovalent association between the VH and VL domains.
  • Clinical and Translational Updates

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