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Your Position: Home > Protein > HLA-A*3303 & B2M & EGF R (HVKITDFGR) > HLR-HP2H3

PE-Labeled Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Tetramer Protein

Streptavidin Protein - PE (STN-NP117, STN-NP119), supporting the assembly of your own MHC Tetramers!

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  • Synonym
    HLA-A*3303 & B2M & EGF R (HVKITDFGR)
  • Source
    PE-Labeled Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Tetramer Protein(HLR-HP2H3) is expressed from human 293 cells (HEK293). It contains AA Gly 25 - Ile 308 (HLA-A*33:03) & Ile 21 - Met 119 (B2M) & HVKITDFGR (Accession # AAA79865.1 (HLA-A*33:03) & P61769-1 (B2M) & HVKITDFGR).
    Predicted N-terminus: Gly 25 & His
  • Molecular Characterization

    PE-Labeled Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Tetramer Protein is assembled by biotinylated monomer and streptavidin.

    Biotinylated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein is produced by co-expression of HLA-A*33:03 and B2M loaded with EGF R peptide. Biotinylated Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Complex Protein carries a polyhistidine tag at the C-terminus, followed by an Avi tag (Avitag™).

    This protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 34.5 kDa & 15.5 kDa.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 1% BSA, 50-150 mM NaCl with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-ELISA
 HLA-A*3303 & B2M & EGF R (HVKITDFGR) ELISA

Immobilized PE-Labeled Human HLA-A*33:03&B2M&EGF R (HVKITDFGR) Tetramer Protein (Cat. No. HLR-HP2H3) at 5 μg/mL (100 μL/well) can bind Monoclonal Anti-B2M Antibody, Human IgG1 with a linear range of 0.1-8 ng/mL (Routinely tested).

  • Background
    The epidermal growth factor receptor (EGFR; ErbB-1; HER1 in humans) is the cell-surface receptor for members of the epidermal growth factor family (EGF-family) of extracellular protein ligands. The epidermal growth factor receptor is a member of the ErbB family of receptors, a subfamily of four closely related receptor tyrosine kinases: EGFR (ErbB-1), HER2/c-neu (ErbB-2), Her 3 (ErbB-3) and Her 4 (ErbB-4). Mutations affecting EGFR expression or activity could result in cancer. The Human HLA-A*3303 & B2M & EGF R(HVKITDFGR) protein is a complex of HLA-A*3303 of the MHC Class I, B2M, and HVKITDFGR peptide of the EGF R.
  • Clinical and Translational Updates

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