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| Assay Type | FACS |
| Analyte | IL-2, IL-4, IL-5, IL-10, TNF-α, IFN-γ |
| Format | 50T |
| Reactivity | Human |
| Regulatory Status | RUO |
| Sensitivity | IL-2 (0.25 pg/mL), IL-4 (0.32 pg/mL), IL-5 (1.15 pg/mL), IL-10 (0.1 pg/mL), TNF-α (0.78 pg/mL), IFN-γ (0.29 pg/mL) |
| Standard Curve Range | 9.8-10000 pg/mL (TNF-α, IFN-γ), 9.8-5000 pg/mL (IL-2, IL-4, IL-5, IL-10) |
| Assay Time | 3 hr |
| Suitable Sample Type | Human serum, Human plasma, Cell culture supernatants |
| Sample volume | 30 μL |
The Human Th1/Th2 Cytokine Kit can be used to quantitatively measure Interleukin-2 (IL-2), Interleukin-4 (IL-4), Interleukin-5 (IL-5), Interleukin-10 (IL-10), Tumor Necrosis Factor-α (TNF-α), and Interferon-γ (IFN-γ) protein levels in a single sample.The kit performance has been optimized for analysis of specific cytokines in tissue culture supernatants, EDTA plasma, and serum samples.
It is for research use only.
The opened kit should be stored per components table. The shelf life is 30 days from the date of opening.
| Catalog | Product Name | Size |
| FMBc007-02 | Anti-human IL-2 Antibody-coupled APC-beads-D01 | 50 tests |
| IL2-PFS426 | PE-Labeled Monoclonal Anti-human IL-2 Antibody, Rabbit IgG | 0.25 mL |
| FMBc008-02 | Anti-human IL-4 Antibody-coupled APC-beads-D05 | 50 tests |
| IL4-PFS269 | PE-Labeled Monoclonal Anti-human IL-4 Antibody, IgG1 | 0.25 mL |
| FMBc014-02 | Anti-human IL-5 Antibody-coupled APC-beads-C03 | 50 tests |
| FABm030-01 | PE labeled Monoclonal Anti-Human IL-5 Antibody, Rat IgG1 | 0.25 mL |
| FMBc017-02 | Anti-human IL-10 Antibody-coupled APC-beads-D08 | 50 tests |
| IL0-PFS428 | PE labeled Monoclonal Anti-Human IL-10 Antibody, Rat IgG1 | 0.25 mL |
| FMBc010-02 | Anti-human TNF-α Antibody-coupled APC-beads-D13 | 50 tests |
| TNA-PFM494 | PE-Labeled Monoclonal Anti-TNF-alpha Antibody, human IgG1 (16H5) | 0.25 mL |
| FMBc011-02 | Anti-human IFN-γ Antibody-coupled APC-beads-D17 | 50 tests |
| IFN-PFM412 | PE-Labeled Monoclonal Anti-IFN-gamma Antibody, human IgG1 (8C5F8) | 0.25 mL |
| FMBf001-02 | APC Positive Control-01 | 0.5 mL |
| FMBf001-01 | PE Positive Control-01 | 0.5 mL |
| FCMB-03 | Assay Buffer-01 | 50 mL |
| FCMB-04 | Wash Buffer-01 | 50 mL |
| FCMP-01 | 96-Well V-Bottom Assay Plate | 1 plate |
| FCMP-02 | 96-well Sealing film | 2 pieces |
| FMBf001-02 | APC Positive Control-01 | 0.5 mL |
| FMBf001-01 | PE Positive Control-01 | 0.5 mL |
A double logarithmic fitting curve was plotted for each cytokine( IL-2, IL-4, IL-5, IL-10, TNF-α, IFN-γ). The present curve of IL-2/IL-4/IL-5/IL-10 was ranging from 9.8 pg/ml to 5000 pg/mL, TNF-α/IFN-γ raging from 9.8 pg/mL to 10000 pg/mL .
A representative scatter plot showing single bead population in P1 gate (left), the beads conjugated with analyte-sepcific antibody differntiated by APC fluorescence intensity (middle), and fluorescence signal in propotion to concentration of the analyte was measured for each cytokine in PE channel (right). Data was generated on Beckman Cytoflex S Flow Cytometer, equipped with blue laser (561 nm) and red laser (640 nm).
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