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Specifically recognizes the target DM-1 & DM-4.
Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) is a Rabbit monoclonal antibody recombinantly expressed from HEK293 cells.
M5D04
Rabbit IgG | Rabbit Kappa
Unconjugated
DM-1-BSA
| Application | Recommended Usage |
| ELISA | 1-500 ng/mL |
Protein A purified / Protein G purified
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Immobilized Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358) at 5 μg/mL, add DM1 Antibody Human IgG in the 100% Human Serum and then add Biotinylated Human Her2, His,Avitag, premium grade (Cat. No. HE2-H82E2) at 0.5 μg/mL. Detection was performed using HRP-conjugated Streptavidin (Acro, Cat. No. STN-NH913) (QC tested).
Immobilized Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358) at 1 μg/mL (100 μL/well) can bind DM1 Antibody Human IgG with a linear range of 0.004-0.125 μg/mL (Routinely tested).
Serial dilutions of DM1 were added into Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358): DM1 Antibody Human IgG binding reactions. The half maximal inhibitory concentration (IC50) is 3.149 μg/mL (Routinely tested).
ELISA binding of Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358) with DM1 Antibody Human IgG, Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and IgG1-MMAF conjugated antibody respectively.
The coating antibody was Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358), used at 1 μg/mL concentration. The primary antibody were different payload conjugated antibodies, including DM1 Antibody Human IgG, Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and IgG1-MMAF conjugated antibodies used at 0.5 μg/mL concentration. The secondary antibody was HRP conjugated Anti-Human-IgG-Fc Antibody (6F11C8), mAb (Acro, Cat. No. IGG-LY69) used at 1:10000 concentration.
Monoclonal Anti-DM-1 Antibody, Rabbit IgG (M5D04) (Cat. No. DM1-MY2358) is specific to DM1 Antibody Human IgG, and has no cross-reactivity with Trastuzumab Deruxtecan, Sacituzumab Govitecam, Trastuzumab MMAE and IgG1-MMAF (Routinely tested).
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