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The kit is developed for quantitative detection of natural and recombinant human Complement C3 in serum, plasma and cell culture supernatants.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | Complement C3 |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Human |
| Sensitivity | 0.2 ng/ml |
| Assay Time | 2.75 hr |
| Sample volume | 50 μL |
| Range | 0.39 ng/mL-25 ng/mL |
| Sample Type | For the quantitative determination of human Complement C3 in serum, plasma and cell supernatant. |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of Complement C3 is less than 0.2 ng/mL.
Twenty four healthy donor serum samples were evaluated for the concentrations of human Complement C3 in assay.
Ten replicates of each of three samples containing different Complement C3 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Three samples containing different concentrations of Complement C3 were tested in independent assays. Acceptable criteria: CV<15%.
Recombinant Complement C3 was spiked into three diluted human serum samples, and then analyzed. The average recovery of Complement C3 for serum samples is 98.9%.
| ID | Components | Size |
| CEA263-C01 | Pre-coated Anti-Complement C3 Antibody Microplate | 1 plate |
| CEA263-C02 | Human Complement C3 Standard | 5 μg×2 |
| CEA263-C03 | Biotin-Anti-Complement C3 Antibody | 1.25 μg |
| CEA263-C04 | Biotin-Antibody Dilution Buffer | 8 mL |
| CEA263-C05 | Streptavidin-HRP Con. Solution | 500 μL |
| CEA263-C06 | Streptavidin-HRP Dilution Buffer | 15 mL |
| CEA263-C07 | 20× Washing Buffer | 50 mL |
| CEA263-C08 | Sample Dilution Buffer | 15 mL×2 |
| CEA263-C09 | Substrate Solution | 12 mL |
| CEA263-C10 | Stop Solution | 6 mL |
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