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The kit is developed for quantitative detection of natural and recombinant human G-CSF in serum, plasma and cell culture supernatants.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | G-CSF |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Human |
| Sensitivity | 1.580 pg/mL |
| Assay Time | 2 hr 20 min |
| Sample volume | 50 μL |
| Range | 6.25 pg/mL-400 pg/mL |
| Sample Type | For the quantitative determination of human G-CSF in serum, plasma and cell supernatant. |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of G-CSF is less than 1.58 pg/mL.
Serum sample: thirty healthy donor serum samples were evaluated for the con-centrations of human G-CSF in assay. The sample detection rate is 36.7%. The range of detected samples is 100.46 pg/mL-55802.16 pg/mL with a mean of 10352.50 pg/mL.
Cell Culture Supernatant: PBMC (5.0×106cells/mL) were cultured in 1640 supple-mented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 ug/mL strep-tomycin sulfate. Cells were cultured unstimulated or stimulated with 0.1ug/mL LPS for 2 days or 5 days. Subsequent analysis involved quantitative measurement of human G-CSF concentrations in collected culture supernatants using standardized immunoassays.
Ten replicates of each of three samples containing different G-CSF concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Three samples containing different concentrations of G-CSF were tested in inde-pendent assays. Acceptable criteria: CV<15%.
Recombinant G-CSF was spiked into three human serum samples, and then ana-lyzed. The average recovery of G-CSF for serum samples is 101.2%.
| ID | Components | Size |
| CEA221-C01 | Pre-coated Anti-G-CSF Antibody Microplate | 1 plate |
| CEA221-C02 | Human G-CSF Standard | 15 μg |
| CEA221-C03 | Biotin-Anti-G-CSF Antibody | 20 μg |
| CEA221-C04 | Streptavidin-HRP | 50 μL |
| CEA221-C05 | 10×Washing Buffer | 50 mL |
| CEA221-C06 | 2×Dilution Buffer | 50 mL |
| CEA221-C07 | Substrate Solution | 12 mL |
| CEA221-C08 | Stop Solution | 7 mL |
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