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Unconjugated Human FcR Protein Panel

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    Components
    Cat. No.Product Name
    FCN-H52W7Human FcRn / FCGRT&B2M Heterodimer Protein, His Tag (SPR & BLI & MALS verified)
    FCA-H52H1Human Fc gamma RI / CD64 Protein, His Tag (SPR & BLI & MALS verified)
    CDA-H5221Human Fc gamma RIIA / CD32a (R167) Protein, His Tag (MALS & BLI verified)
    CD1-H5223Human Fc gamma RIIA / CD32a (H167) Protein, His Tag (SPR & BLI & MALS verified)
    CDB-H5228Human Fc gamma RIIB/C (CD32b/c) Protein, His Tag (SPR & BLI & MALS verified)
    CD8-H52H4Human Fc gamma RIIIA / CD16a (V176) Protein, His Tag (SPR & BLI & MALS verified)
    CDA-H5220Human Fc gamma RIIIA / CD16a (F176) Protein, His Tag (MALS & SPR verified)
    CDB-H5227Human Fc gamma RIIIB / CD16b (NA1) Protein, His Tag (SPR & BLI & MALS verified)
    CDB-H5222Human Fc gamma RIIIB / CD16b (NA2) Protein, His Tag (SPR & BLI & MALS verified)
  • Features

    Comprehensive FcRs Protein Panel – No separate purchasing needed
    High Purity – >95% by SDS-PAGE; >90% by SEC-MALS
    Robust Stability – High batch-to-batch consistency
    Full Data Package – SPR, BLI, and ELISA activity data included

  • Purity

    >95% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-SPR
 - SPR

Captured Human FCGRT&B2M Heterodimer Protein, His Tag (Cat. No. FCN-H52W7) on CM5 Chip via Anti-His antibody can bind Herceptin® (Trastuzumab) with an affinity constant of 0.516 μM as determined in a SPR assay (Biacore T200) (QC tested).

 - SPR

Human CD64, His Tag (Cat. No. FCA-H52H1) captured on CM5 chip via anti-His antibody can bind Herceptin® with an affinity constant of 4.92 nM as determined in a SPR assay (Biacore 8K) (QC tested).

 - SPR

Immobilized Human CD32a (R167), His Tag (Cat. No. CDA-H5221) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 3.12 μM as determined in a SPR assay (Biacore T200) (QC tested).

 - SPR

Immobilized Human CD32a (H167), His Tag (SPR & BLI verified) (Cat. No. CD1-H5223) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 1.45 μM as determined in a SPR assay (Biacore T200) (QC tested).

 - SPR

Immobilized Human CD32b/c, His Tag (Cat. No. CDB-H5228) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 15.1 μM as determined in a SPR assay (Biacore T200) (QC tested).

 - SPR

Immobilized Human CD16a (V176), His Tag (Cat. No. CD8-H52H4) on CM5 Chip via anti-His antibody, can bind Herceptin with an affinity constant of 0.463 μM as determined in SPR assay (Biacore T200) (QC tested).

 - SPR

Human CD16a (F176), His Tag (Cat. No. CDA-H5220) captured on CM5 chip via anti-His antibody can bind Rituximab biosimilar (Cat. No. CD0-M36) with an affinity constant of 1.18 µM as determined in a SPR assay (Biacore 8K) (QC tested).

 - SPR

Immobilized Human CD16b (NA1), His Tag (Cat. No. CDB-H5227) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 8.17 μM as determined in a SPR assay (Biacore T200) (QC tested).

 - SPR

Immobilized Human CD16b (NA2), His Tag (Cat. No. CDB-H5222) on CM5 Chip via anti-His antibody, can bind Rituximab with an affinity constant of 2.88 μM as determined in a SPR assay (Biacore T200) (QC tested).

  • Clinical and Translational Updates

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