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Your Position: Home > Protein > FGF-10 > FG0-H5147

Human FGF-10 / KGF 2 Protein, His Tag, premium grade

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  • Synonym
    FGF-10,Fibroblast growth factor 10,Keratinocyte growth factor 2
  • Source
    Human FGF-10 Protein, His Tag, premium grade(FG0-H5147) is expressed from E. coli cells. It contains AA Gln 38 - Ser 208 (Accession # O15520-1).
    Predicted N-terminus: Met
    It is produced under our rigorous quality control system that incorporates a comprehensive set of tests including sterility and endotoxin tests. Product performance is carefully validated and tested for compatibility for cell culture use or any other applications in the early preclinical stage. When ready to transition into later clinical phases, we also offer a custom GMP protein service that tailors to your needs. We will work with you to customize and develop a GMP-grade product in accordance with your requests that also meets the requirements for raw and ancillary materials use in cell manufacturing of cell-based therapies.
  • Molecular Characterization
    FGF-10 Structure

    This protein carries a polyhistidine tag at the N-terminus.

    The protein has a calculated MW of 21.3 kDa. The protein migrates as 25-27 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Endotoxin
    Less than 0.01 EU per μg by the LAL method / rFC method.
  • Sterility
    Negative
  • Mycoplasma
    Negative
  • Purity

    >95% as determined by SDS-PAGE.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.2 M Arginine, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
FGF-10 SDS-PAGE

Human FGF-10 Protein, His Tag, premium grade on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95% (With Star Ribbon Pre-stained Protein Marker).

Bioactivity-ELISA
 FGF-10 ELISA

Immobilized Human FGF-10 Protein, His Tag, premium grade (Cat. No. FG0-H5147) at 5 μg/mL (100 μL/well) can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with a linear range of 0.1-6 ng/mL (QC tested).

Bioactivity-SPR
 FGF-10 SPR

Human FGF-10 Protein, His Tag, premium grade (Cat. No. FG0-H5147) immobilized on CM5 Chip can bind Human FGF R2 (IIIb), Fc Tag (Cat. No. FGB-H5256) with an affinity constant of 4.11 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).

  • Background
    Fibroblast Growth Factor 10 (FGF 10) is an evolutionary conserved secreted growth factor mediating mostly mesenchymal to epithelial signaling. FGF 10 belongs to the FGF 7 subfamily and shares similar biochemical and amino acid sequences with its constituent members (FGF3, FGF 7 and FGF 22). As a paracrine FGF, FGF 10 elicits its biological responses by activating the fibroblast growth factor receptor 2b (FGF R 2b), is crucial for governing proximal distal outgrowth as well as patterning and acts upstream of the known apical ectodermal ridge (AER) marker FGF 8. FGF10 is also implicated in pancreatic cancer, and that overexpression of FGFR2b is associated with metastatic invasion.
  • Clinical and Translational Updates

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