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| Items | Specifications |
| Material | Polystyrene |
| Color | Clear |
| Plate Blocking | 2% BSA Blocking Buffer |
| Formulations | Clear, 96-well plates, coated with 100uL of Streptavidin and blocked with 200uL of 2% BSA Blocking Buffer, Dilution Buffer 50 mL |
| Detection Method | Colorimetric |
| Capacity | ~5 pmol biotin/well |
| CV% of plates/wells | < 10% |
| Type | Detection Plate, Immunoassay, ELISA |
Streptavidin (SA) has an extraordinarily high affinity for biotin with a dissociation constant (Kd) on the order of 10−14 mol/L, the Biotinylated molecules can bind to the SA irreversibly. Streptavidin has an isoelectric point of 5 to 6, resulting in low nonspecific interactions. The Streptavidin Coated Plates we provide are easy to use and widely available for applications. Example ELISA Procedure.
Immobilized Biotinylated SARS-CoV-2 S protein RBD, His, Avi tag at 1000 ng/mL (100 μL/well) on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (For Serological Testing) (Cat. No. SP-13), can bind Anti-SARS-CoV antibody in serum with a linear range of 0.1-3 ng/mL (Routinely tested). The minimum OD is about 0.15.
Immobilized 0.1μg biotinylated protein on Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (For Serological Testing) (Cat. No. SP-13), the signal of detection antibody in serum were similar with other two plates which claim to reduce background.
There were no signal between detection antibody with no biotin in serum and Streptavidin Coated Plates, Clear, 12×8-Well Strips, White Frame (For Serological Testing) (Cat. No. SP-13), but competitors were positive with some antibodies.
Cat. No. SP-13, Adsorption stability test of avidin protein. After three SA plates were washed with buffer solution for three times, five times and ten times respectively, binding activity between the SA plate, the Spike RBD Protein and the Anti-SARS-CoV-2 Spike RBD Antibody showed no difference. The test shows that the SA protein has good stability on the plate.
Emerging VoCs, Omicron, Delta, Beta, Alpha mutants and so on, including RBD, S trimer, S1, NTD, NP, etc. These mutants are of high purity and bioactivity and can be used to evaluate the efficacy of the antibodies and vaccination.
ACROBiosystems developed a series of GMP grade cytokines under the GMP grade quality management system. Those products are all suitable for T/NK cell generation, activation, and proliferation in cell therapy research.
50+ targets designed for CAR detection, including PE/FITC/biotin labeled proteins. The key reagents for CD19 and BCMA were FDA DMF filed which can support your IND, NDA and BLA process.
GMP grade cytokines, reagents for cell activation, gene edition, DNA/RNA removal, etc. Particularly focus on product design, quality control and solution-based support to link each phase of your cell and gene therapy journey.
Full length multi-pass TPs with stabilized structure and high bioactivity for immunization, antibody screening, cell based assay and CAR detection, including hot CD20, Claudin 18.2， CD133, GPRC5D，CCR8, CCR5, etc.
CD3 proteins and a collection of for bispecific antibody development which are of high specificity and bioactivities and suitable for immunization, antibody screening.
A series of immune checkpoints including classic co-inhibitory and co-stimulatory receptors. The comprehensive catalog contains 100+ targets with various species and tags, and the high-quality proteins are in good batch-to-batch consistency.
To meet the needs of ADCs development, ACROBiosystems can provide: A variety of high-quality target proteins; MMPs/Cathepsin/uPA for cleavable linker; Anti-payload antibodies & anti-idiotypic antibodies for immunogenicity and PK analysis; SPR/BLI analytical and ADA development service.
Comprehensive collection of Fc receptor proteins, including their common variants, which can help expedite your antibody development.
The MABSOL biotinylated protein collection includes more than a hundred commonly studied drug targets and biomarker proteins.
Comprehensive cytokines including IL families, growth factors, chemokines, TNFs, etc. These products are HEK293 expressed and nearly in authentic structure, high purity and bioactivity, cell based assay/SPR/BLI verified.
To support preclinical/clinical immunogenicity and PK analysis, ACROBiosystems has developed a series of high-affinity anti-idiotypic antibodies. Our pipeline covers five hot targets including adalimum*b, rituxim*b, cetuxim*b, trastuzum*b, and bevacizum*b.
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