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Your Position: Home > Protein > TSLP > TSP-HP2H4

PE-Labeled Human TSLP Protein, His Tag (Site-specific conjugation)

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  • Synonym
    TSLP
  • Source
    PE-Labeled Human TSLP Protein, His Tag(TSP-HP2H4) is expressed from human 293 cells (HEK293). It contains AA Tyr 29 - Gln 159 (Accession # Q969D9-1).
    Predicted N-terminus: Tyr 29
  • Molecular Characterization
    TSLP Structure

    This protein carries a polyhistidine tag at the C-terminus.

    The protein has a calculated MW of 18.6 kDa.

  • Conjugate

    PE

    Excitation Wavelength: 488 nm / 561 nm

    Emission Wavelength: 575 nm

  • Formulation

    Lyophilized from 0.22 μm filtered solution in PBS, 0.5% BSA, pH7.4 with trehalose as protectant.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please protect from light and avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
Bioactivity-FACS
 TSLP FACS

5e5 of Human TSLP R (Luc) HEK293 Reporter Cells were stained with 100 μL of 1:50 dilution (2 μL stock solution in 100 μL FACS buffer) of PE-Labeled Human TSLP Protein, His Tag (Cat. No. TSP-HP2H4) and negative control protein respectively. PE signal was used to evaluate the binding activity (QC tested).

  • Background
    Thymic stromal lymphopoietin (TSLP) is an epithelial cell-derived cytokine involved in the pathology of inflammatory skin diseases, and is widely expressed by epithelial cells. Human TSLP cDNA encodes a 159 amino acid (aa) residue precursor protein with a 28 aa signal sequence (4, 5). Human TSLP has been shown to developing nondeletional central tolerance, amplifying epithelium-induced class switching, inducing atopic diseases and maintaining intestinal noninflammatory environment. Among diverse cells responding to Human TSLP, CD11c+ dendritic cells are the most obviously characterized target cells.
  • Clinical and Translational Updates

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