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This protein carries an Avi tag (Avitag™) at the N-terminus, followed by a polyhistidine tag.
The protein has a calculated MW of 44.1 kDa. The protein migrates as 11-12 kDa, 40-43 kDa and 55 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE) due to glycosylation.
>90% as determined by SDS-PAGE.
Lyophilized from 0.22 μm filtered solution in PBS, pH7.4 with trehalose as protectant.
Contact us for customized product form or formulation.
Please see Certificate of Analysis for specific instructions.
For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.
For long term storage, the product should be stored at lyophilized state at -20°C or lower.
Please avoid repeated freeze-thaw cycles.
This product is stable after storage at:
Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).
Immobilized Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Apitegromab with a linear range of 0.06-1 ng/mL (QC tested).
Immobilized Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) at 1 μg/mL (100 μL/well) on streptavidin (Cat. No. STN-N5116) precoated (0.5 μg/well) plate can bind Human Activin RIIB Protein, Fc Tag (Cat. No. ACB-H5254) with a linear range of 0.002-0.313 μg/mL (Routinely tested).
Immobilized Human Follistatin Protein, His Tag, premium grade (Cat. No. FON-H52H4) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) with a linear range of 0.5-16 ng/mL (Routinely tested).
Immobilized Human Activin RIIB Protein, Fc Tag (Cat. No. ACB-H5254) at 5 μg/mL (100 μL/well) can bind Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) with a linear range of 0.01-0.313 μg/mL (Routinely tested).
Apitegromab captured on Protein A Chip can bind Biotinylated Cynomolgus latent GDF-8 Protein, Avitag,His Tag (Cat. No. GD8-C82Q3) with an affinity constant of 25.1 nM as determined in a SPR assay (Biacore 8K) (Routinely tested).
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