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Your Position: Home > Protein > Streptavidin > STN-N5113

Recombinant Streptavidin Protein (MALS verified)

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  • Synonym
    Streptavidin,SA
  • Source
    Recombinant Streptavidin Protein(STN-N5113) is expressed from E. coli cells. It contains AA Ala 37 - Ser 163 (Accession # P22629-1).
    Predicted N-terminus: Met
  • Molecular Characterization
    Streptavidin Structure

    This protein carries no "tag".

    The protein has a calculated MW of 13.4 kDa. The protein migrates as 11-12 kDa when calibrated against Star Ribbon Pre-stained Protein Marker under reducing (R) condition (SDS-PAGE).

  • Purity

    >90% as determined by SDS-PAGE.

    >90% as determined by SEC-MALS.

  • Formulation

    Lyophilized from 0.22 μm filtered solution in 0.02% NaCl, pH7.3.

    Contact us for customized product form or formulation.

  • Reconstitution

    Please see Certificate of Analysis for specific instructions.

    For best performance, we strongly recommend you to follow the reconstitution protocol provided in the CoA.

  • Storage

    For long term storage, the product should be stored at lyophilized state at -20°C or lower.

    Please avoid repeated freeze-thaw cycles.

    This product is stable after storage at:

    1. -20°C to -70°C for 12 months in lyophilized state;
    2. -70°C for 3 months under sterile conditions after reconstitution.
SDS-PAGE
Streptavidin SDS-PAGE

Recombinant Streptavidin Protein on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 90% (With Star Ribbon Pre-stained Protein Marker).

SEC-MALS
Streptavidin MALS images

The purity of Recombinant Streptavidin Protein (Cat. No. STN-N5113) is more than 90% and the molecular weight of this protein is around 45-65 kDa verified by SEC-MALS.

Bioactivity-ELISA
 Streptavidin ELISA

Immobilized Recombinant Streptavidin Protein (Cat. No. STN-N5113) can bind Biotinylated Human PVRIG, Fc,Avitag (Cat. No. PVG-H82F4) at 1 μg/mL (100 μL/well) with a linear range of 0.2-6 ng/mL (QC tested).

  • Background
    Streptavidin is a 66KDa tetrameric protein purified from the bacterium Streptomyces avidinii, and exhibits high binding affinity to biotin. Each unit can bind one biotin. Horseradish peroxidase is metalloenzyme, a 44KDa glycoprotein. When incubate with substrates, it produces a coloured, fluorimetric, or luminescent derivatives, which can be detected and quantified. HRP conjugated Streptavidin is widely used for the detection and quantification of biotinylated proteins.
  • Clinical and Translational Updates

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