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The kit is developed for quantitative detection of natural and recombinant human IL-11 in serum, plasma and cell culture supernatants.
It is for research use only.
| Assay Type | Sandwich-ELISA |
| Analyte | IL-11 |
| Format | 96-wells plate breakable into 12 x 8 wells strips |
| Reactivity | Human |
| Sensitivity | 0.335 pg/mL |
| Assay Time | 3 hr 20 min |
| Sample volume | 50 μL |
| Range | 3.906 pg/mL-250 pg/mL |
| Sample Type | For the quantitative determination of human IL-11 in serum, plasma and cell supernatant. |
Elevate your research experience with our Cytokine/Biomarker Detection Kits, where accuracy, reliability, and ease of use are converging to deliver exceptional results.
For each experiment, a standard curve needs to be set for each microplate, and the specific OD value may vary depending on different laboratories, testers, or equipment. The following example data is for reference only. The sample concentration was cal-culated based on the results of the standard curve. The minimum detectable con-centration of IL-11 is less than 0.335 pg/mL.
MRC-5 Medical Research Council cell strain-5 (1.0×106cells/mL) were cultured in DMEM supplemented with 10% fetal bovine serum, 100 U/mL penicillin, and 100 μg/mL streptomycin sulfate. Cells were cultured unstimulated or stimulated with 30ng/mL IL-1β and 30ng/mL TNF-α for 2 days. Subsequent analysis involved quantitative measurement of human IL-11 concentrations in collected culture supernatants using standardized immunoassays.
Ten replicates of each of three samples containing different IL-11 concentrations were tested in one assay. Acceptable criteria: CV＜10%.
Three samples containing different concentrations of IL-11 were tested in inde-pendent assays. Acceptable criteria: CV<15%.
Recombinant IL-11 was spiked into three human serum samples, and then ana-lyzed. The average recovery of IL-11 for serum samples is 89.5%.
| ID | Components | Size |
| CEA215-C01 | Pre-coated Anti-IL-11 Antibody Microplate | 1 plate |
| CEA215-C02 | Human IL-11 Standard | 20 μg |
| CEA215-C03 | Biotin-Anti-IL-11 Antibody | 15 μg |
| CEA215-C04 | Streptavidin-HRP | 50 μL |
| CEA215-C05 | 10×Washing Buffer | 50 mL |
| CEA215-C06 | 2×Dilution Buffer | 50 mL |
| CEA215-C07 | Substrate Solution | 12 mL |
| CEA215-C08 | Stop Solution | 7 mL |
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